NR1H4 Knockout cell line(Hep 3B)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

NR1H4 Knockout cell line(Hep 3B) Order

Catalog#

YKO-H066

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

NR1H4 Knockout cell line (Hep 3B)

Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

NR1H4

Gene ID

9971

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

87%MEM+10% FBS+1%Glutamax+1%Sodium Pyruvate+1%NEAA

Target region

Knockout region: Exon 4 (based on transcript NR1H4-203)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Hep 3B			STR Info (Cell bank) Cell Line:HEP-3B
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	12	14		12	14
D7S820	8	10		8	10
D16S539	10	10		10	10
VWA	17	17		17	17
TH01	6	7		6	7
AMEL	X	X		X	X
TPOX	9	9		9	9
CSF1PO	8	8		8	8

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NR1H4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep 3B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NR1H4 gene knockout NR1H4 Knockout cell line (Hep 3B) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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